Device
IR-GFP

Part:BBa_K658016

Designed by: Yidan Hu   Group: iGEM11_XMU-China   (2011-10-02)
Revision as of 06:56, 2 October 2011 by Registry (Talk | contribs)

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LuxI->LuxR->lux pR->GFP

This device is made up of three parts: a LuxI producer, a Lux R producer and a GFP reporter. It is used to test the efficiency of promoter lux pR(BBa_R0062) and its mutants lux pR-3 (BBa_K658006), lux pR-5 (BBa_K658007), lux pR-3/5 (BBa_K658008).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1874
    Illegal BsaI.rc site found at 2601


[edit]
Categories
Parameters
controlR0011
proteinsLuxI LuxR GFP
signalling_moleculeAHL