Plasmid_Backbone

Part:pSB6A1:Experience

Designed by: Stefan Luzi   Group: iGEM06_ETHZ   (2007-10-23)
Revision as of 16:23, 1 October 2011 by Emichael (Talk | contribs) (→‎User Reviews)

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Applications of pSB6A1

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This plasmid has been adapted to the version 5 of BioBrick vector backbones by the ETHZ 2011 iGEM team. This means, that the cassettes for the ORI and antibiotic resistance have been minimized (reduction of the size and transcriptional terminators flanking the prefix and the suffix have been added, to trancriptionally insulate the inserted parts from the vector backbone machinery and vice versa. This minimization yielded a reduction in size from 4022 bp to 2743 bp.

For comparison of the copy number of the plasmids, triplicates of OD normalized bacterial cultures containing the respective plasmids were miniprepped. The DNA concentration was then determined by an agarose DNA gel electrophoresis of EcoRI digests of the purified plasmids. This experiment showed a clear reduction of

Figure 1: Agarose gel showing the comparison of the .


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