Regulatory
Pu

Part:BBa_I723020:Experience

Designed by: Scott Ramsay   Group: iGEM07_Glasgow   (2007-10-25)
Revision as of 15:55, 1 October 2011 by Luwidmer (Talk | contribs)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I723020

User Reviews

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BBa_I723020 1 Not understood Sebastian Murmann ETH Zürich 2011

We had some troubles to get the part out of the iGEM spring distribution 2011. Although trying for several times, we did not succeed in transforming the plasmid provided in the distribution. Thus we used the plasmid pCK04YxylR as source for the promoter. We further modified the part by adding a double stop codon to it, resulting in BBa_K625002. The second modification was to remove the remaining sequence of xylU, which resulted in the short promoter variant BBa_K625003. The characterization and comparison of the new promoters can be found in the characterization section of BBa_K625002 respectively BBa_K625003.

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