Plasmid_Backbone

Part:pSB6A1:Experience

Designed by: Stefan Luzi   Group: iGEM06_ETHZ   (2007-10-23)
Revision as of 15:48, 1 October 2011 by Smurmann (Talk | contribs) (→‎User Reviews)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of pSB6A1

User Reviews

UNIQ7c8ad37d887822c6-partinfo-00000000-QINU

No review score entered. Username

This plasmid has been adapted to the version 5 of BioBrick vector backbones by the ETHZ 2011 iGEM team. This means, that the cassettes for the ORI and antibiotic resistance have been minimized (reduction of the size and transcriptional terminators flanking the prefix and the suffix have been added, to trancriptionally insulate the inserted parts from the vector backbone machinery and vice versa. This minimization yielded a reduction in size from 4022 bp to 2743 bp.

For comparison of the copy number of the plasmids, triplicates of OD normalized bacterial cultures containing the respective plasmids were miniprepped and the DNA concentration determined by an agarose DNA gel of a dilution series of an EcoRI digest. This experiment showed a clear reduction of

;

UNIQ7c8ad37d887822c6-partinfo-00000002-QINU