Regulatory

Part:BBa_K658008:Design

Designed by: Yidan Hu   Group: iGEM11_XMU-China   (2011-10-01)
Revision as of 13:29, 1 October 2011 by Registry (Talk | contribs)

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position 3&5 mutated promoter lux pR-3/5 (luxR & HSL regulated)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have generated two point mutants by site-directed mutagenesis from the sequence of lux pR(BBa_R0062). In these mutants, the G residue of position 5 was changed to a C and the C residue of position 3 was changed to a T respectively.


Source

The promoter lux pR (BBa_R0062).

References