Regulatory

Part:BBa_J64010:Experience

Designed by: Jeffrey J Tabor   Group: Voigt Lab   (2007-01-15)
Revision as of 12:22, 1 October 2011 by Notothenia (Talk | contribs) (→‎Applications of BBa_J64010)

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Applications of BBa_J64010

Fluorescence intensity of BBa_K649001 was not increased by 3O-C12-HSL induction.


Generally, in the presence of 3O-C12-HSL, lasR activates lasI promoter and the transcription level of downstream gene increases, but in the absence of 3O-C12-HSL, lasR can't activate lasI promoter. To characterize BBa_J64010, we used PlasI(BBa_J64010)-rbs-gfp-TT on pSB3K3 as reporer part and Ptrc-rbs-lasR-TT on pBR as regulator part.

Since Ptrc is a constitutive promoter, lasR is constitutively expressed and, since it is know that this lasR is a working part, the fact that the fluorescence intensity levels do not change before and after induction of BBa_K649000 by 3O-C12-HSL indicates that BBa_K649000 is not regulated by 3O-C12-HSL.

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