Coding
leuB

Part:BBa_K594002:Design

Designed by: Yu Xiaoyan   Group: iGEM11_OUC-China   (2011-09-30)
Revision as of 23:45, 30 September 2011 by Registry (Talk | contribs)

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subunit of a 3-isopropylmalate dehydrogenase from E.coli BL21


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Besides, when we were looking for leuB gene, we also try to find its RBS, but unluckily, we didn't find its RBS on NCBI, its location is closely linked to leuA's location.


Source

This leuB is from the leu operon in BL 21(E.coli),we get BL21 from Takara and found its sequence in NCBI, then cloned it with a Pfu PCR.

References