Composite

Part:BBa_K567001:Design

Designed by: Chunying Li and Yunfeng Ruan   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 15:41, 30 September 2011 by Cathy zks (Talk | contribs) (→‎Design Notes)

lacI-Ptrc-tRNA(Arg)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1845
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1845
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1845
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1845
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2026


Design Notes

The correct processing to produce mature tRNA should be ensured.

Source

LacI- Ptac derived from plasmid pTrc99B. tRNA(Arg) from E.coli ArgW operon.

References