Translational_Unit
HO1
Part:BBa_K646000:Design
Designed by: Edward Moh Group: iGEM11_Macquarie_Australia (2011-09-29)
Hemeoxygenase with RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene was digested with XbaI and SpeI for the BioBrick construction due to the primer design, and the gene orientation was screened by an XbaI digest of the constructed BioBrick. For subsequent usage of this part, iGEM BioBrick assembly protocol could be used; digest using either EcoRI with SpeI or XbaI with PstI.
Source
Http://www.ncbi.nlm.nih.gov/gene/953483