Device

Part:BBa_K676004:Design

Designed by: Ejaj Intisar   Group: iGEM11_UCL_London   (2011-09-11)
Revision as of 10:31, 30 September 2011 by Eintisar (Talk | contribs) (→‎References)

Supercoilometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter).

UCL-Content-Experiment Plan for Supercoilometer.jpg

Source

TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M).

References

1. Straney, R., R. Krah, and R. Menzel, Mutations in the -10 Tataat Sequence of the Gyra Promoter Affect Both Promoter Strength and Sensitivity to DNA Supercoiling. Journal of Bacteriology, 1994. 176(19): p. 5999-6006. 2. Pemberton, I.K., et al., The G+C-rich discriminator region of the tyrT promoter antagonises the formation of stable preinitiation complexes. Journal of Molecular Biology, 2000. 299(4): p. 859-864. 3. Lamond, A.I., Supercoiling response of a bacterial tRNA gene. Embo Journal, 1985. 4(2): p. 501-7. 4. Bowater, R.P., D.R. Chen, and D.M.J. Lilley, Modulation of Tyrt Promoter Activity by Template Supercoiling in-Vivo. Embo Journal, 1994. 13(23): p. 5647-5655. 5. El Hanafi, D. and L. Bossi, Activation and silencing of leu-500 promoter by transcription-induced DNA supercoiling in the Salmonella chromosome. Molecular Microbiology, 2000. 37(3): p. 583-594. 6. Bossi, L., et al., The supercoiling sensitivity of a bacterial tRNA promoter parallels its responsiveness to stringent control. Embo Journal, 1998. 17(8): p. 2359-2367.