Translational_Unit

Part:BBa_K567013

Designed by: Bin Zhao   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 04:38, 30 September 2011 by Registry (Talk | contribs)

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tRNA(Asp)-TAG

tRNA(Asp) with its anticodon mutated to CUA (base pairing stop codon UAG) and under the control of lpp promoter. This biobrick is constructed first by cloning the tRNA(Asp) from AspV in E.coli, then the anticodon region is site-directed mutated.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 281
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 281
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 281
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 281
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 281
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
chassisEscherichia coli