Translational_Unit

Part:BBa_K567011

Designed by: Bin Zhao   Group: iGEM11_SJTU-BioX-Shanghai   (2011-09-29)
Revision as of 04:28, 30 September 2011 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


PT7-TDRS

aspartyl aminoacyl tRNA synthetase without anticodon recognition domain under the control of T7 promoter and lac operator. This biobrick is constructed by deleting the anticodon recognition domain of AspRS from E.coli. This modified AspRS can charge Asp to tRNA(Asp)-TAG (BBa_K567013) and tRNA(Asp)-AGG (BBa_K567012).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal NheI site found at 1556
    Illegal PstI site found at 1152
    Illegal NotI site found at 1620
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal BamHI site found at 1589
    Illegal XhoI site found at 1629
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1595
    Illegal EcoRI site found at 1767
    Illegal XbaI site found at 48
    Illegal PstI site found at 1152
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/enzyme
Parameters
chassisER2566
functionanimo acylate tRNA(Asp)
ligandsIsopropyl β-D-1-Thiogalactopyranoside (IPTG)
n/aPT7-TDRS