Translational_Unit
AT-Bph

Part:BBa_K646001

Designed by: David Dubow   Group: iGEM11_Macquarie_Australia   (2011-09-29)
Revision as of 03:25, 30 September 2011 by BlueDave (Talk | contribs)

Agrobacterium tumefaciens Bacteriophotochrome (Rhizobium radiobacter)

The Agrobacterium tumefaciens bacteriophytochrome binds to biliverdin, a chromophore. Biliverdin is produced in the heme metabolic pathway catalysed by heme oxygenase. Once bound to the chromophore the complex can absorb red and far red light causing the complex to change colour from blue to green reversibly. There is a ribosome binding site six bases upstream from the ATG start codon. Sequence from National Center for Biotechnology.

This part was made using BBa_K497004 and PCR.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1867
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1694
    Illegal AgeI site found at 1759
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None