Device
Part:BBa_K533003
Designed by: ZHANG Yunxiao Group: iGEM11_Tsinghua (2011-09-29)
OmpA-HIV aspartyl protease
OmpA is used to present HIV aspartyl protease outside the E. Coli membrane so that it can cut other proteins with the specific sequence outside in the media.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 936
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//cds/enzyme/protease
//cds/membrane/extracellular
//plasmid/expression/t7
//cds/membrane/extracellular
//plasmid/expression/t7
negative_regulators | Temperature < 20 centigrade for efficient protein folding |
positive_regulators | IPTG, 20mM |