Part:BBa_K672001:Design

New Promoter For Periplasmic Heat Generation

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 322
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

As the part K328000 is a basic part, instead of a combination of subparts (Promoter, etc...), we had to analyse the sequence, cut out the HybB promoter and combine manually with the CsPA sequence.

Source

https://parts.igem.org/Part:BBa_K410000 (hybB-ompA-AOX1A)
https://parts.igem.org/Part:BBa_K328001 (CsPA promoter)

CsPA Promoter

• Fang L., Jiang W., Bae W., Inouye M., 1997, Promoter-independent cold-shock induction of cspA and its derepression at 37 degrees C by mRNA stabilization., Mol Microbiol. 23(2):355-64
  
• Hankins JS., Zappavigna C., Prud'homme-Genereux A., Mackie GA., 2007, Role of RNA structure and susceptibility to RNase E in regulation of a cold shock mRNA, cspA mRNA., J Bacteriol. 189(12):4353-8
  
• Xia B., Ke H., Jiang W., Inouye M., 2002, The Cold Box stem-loop proximal to the 5'-end of the Escherichia coli cspA gene stabilizes its mRNA at low temperature., J Biol Chem. 277(8):6005-11

Aox

• Grant, N et al. “Two Cys or Not Two Cys? That is the Question; Alternative Oxidase in the Thermogenic Plant sacred Lotus.” Plant Physiology 150 (2009): 987-995. {PAPER}
• OmpA Sequence:http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/e8283dat.Par.0001.File.tmp/e8283dat.pdf
• AoxA Sequence:http://www.ncbi.nlm.nih.gov/nuccore/226897260#sequence_226897260