Part:BBa_K672000:Experience

Experiments with BBa_K672000

We did Spectrophotometry measurments, obtaining the following results:

Which leads us to the following conclusions:

1. The best growth is obtained in Luria Broth.
   
2. Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.

Experiments with Other BioBricks

BBa_K381001 Bristol

Sample ID Temperature (°C) Substrate Absorbance @420 nm BBa_K381001 Bristol 23 LB 0.599 BBa_K381001 Bristol 37 LB 0.961 BBa_K381001 Bristol 8 LB 0.015 BBa_K381001 Bristol 23 MM 0.341 BBa_K381001 Bristol 37 MM 0.351 BBa_K381001 Bristol 8 MM 0.021 BBa_K381001 Bristol 23 SS 0.010 BBa_K381001 Bristol 37 SS 0.006 BBa_K381001 Bristol 8 SS 0.005

From these data we could conclude that: • The BB grows best in LB. • It grows less in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows good @23°C • It shows almost no growth @8°C

BBa_K410000 GaTech

Sample ID Temperature (°C) Substrate Absorbance @420 nm BBa_K410000 GaTech 23 LB 0.258 BBa_K410000 GaTech 37 LB 0.624 BBa_K410000 GaTech 8 LB 0.006 BBa_K410000 GaTech 23 MM 0.275 BBa_K410000 GaTech 37 MM 0.028 BBa_K410000 GaTech 8 MM 0.004 BBa_K410000 GaTech 23 SS 0.015 BBa_K410000 GaTech 37 SS -0.001 BBa_K410000 GaTech 8 SS 0.014

From these data we could conclude that: • The BB grows best in LB. • It grows well in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows well @23°C (best in Minimal Media than LB) • It shows almost no growth @8°C

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