Composite

Part:BBa_K575024

Designed by: Kristin Palarz   Group: iGEM11_Northwestern   (2011-09-22)
Revision as of 05:14, 29 September 2011 by Nirmitd7 (Talk | contribs) (Undo revision 119104 by Nirmitd7 (Talk))

This construct was developed by Northwestern's 2011 iGEM team as part of a Pseudomonas Aeruginosa detector. The device is designed to fluoresce with GFP in the presence of PAI1 (3-oxo-C12-HSL), one of the Pseudomonas quorum sensing molecules. The promoter in front of GFP is activated by the combination of PAI1 from the environment and the LasR receptor (produced by this construct).

Standardized Fluorescence for S1.jpg

LasR/PAI1 Inducible Promoter + RBS (B0030) + GFP, Constitutive Promoter + RBS (B0030) + LasR

Continuous expression of LasR (with RBS B0030), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0030), and a GFP reporter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 921
    Illegal NheI site found at 944
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1300
    Illegal AgeI site found at 1497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 830


[edit]
Categories
Parameters
n/aLasP/30/G +CP/30/LasR