Regulatory

Part:BBa_K619893:Design

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-27)
Revision as of 04:33, 29 September 2011 by Jgrose (Talk | contribs) (→‎Design Notes)

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mutated prfA-UTR thermosensor from Listeria monocytogenes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Our "wild-type" thermosensor (BBa-19889) Shine-Delagarno has been optimized for expression in E. coli. Otherwise, the "wild-type" sequence is identical to the thermosensor sequence from Listeria monocytogenes. Sequencing of this mutant revealed a single T56C bp change.

Source

prfA-UTR from Listeria monocytogenes

References