Regulatory

Part:BBa_K619889:Design

Designed by: Julianne Grose   Group: iGEM11_BYU_Provo   (2011-09-26)
Revision as of 04:18, 29 September 2011 by Jgrose (Talk | contribs) (→‎Design Notes)

prfA-UTR, RNA thermosensor from Listeria monocytogenes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them. We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA. This should not have a drastic affect the hairpin structure since it is in an unpaired region.

Source

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.

References