Part:BBa_K643000
CDS7 cadmium sulfate binding peptide coding sequence
The protein coded by this part is claimed to be capable of binding to cadmium chloride and sodium sulfide, forming a cadmium sulfate nanocrystal that exhibits quantum dot behavior.
Experience
We've only had a short time working with the sequence due to difficulties associated with smaller basepair number segment. We followed the protocols outlined in the original reference paper and then ran the results through fluorescent microscopy for confirmation
Result
Picture from one of the microscopy runs. Cells exhibit fluorescent behavior from green to red but negative controls exposed to cadmium and sulfide mixture without engineered genetic sequence showed similar, if a little less, amount of fluorescence.
Future
We plan to carry on the experiment even after the iGEM and complete quantum dot producing system for future iGEM projects
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