Regulatory

Part:BBa_K640001:Design

Designed by: Emily Hicks   Group: iGEM11_Calgary   (2011-09-25)
Revision as of 03:55, 29 September 2011 by Emily Hicks (Talk | contribs)

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Hsp70A/RbcS2 Promoter from Chlamydomonas reinhardtii


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 252
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 655
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was originally received from the chlamydomonas database (chlamydb.org). This part contained no illegal cut sites and the Biobrick prefix and suffix were added via polymerase chain reaction.


Source

http://www.chlamy.org/chlamydb.html

References

Fuhrmann et al. (2004) 

  Plant Mol. Biol. 55, 869-881

Heitzer and Zschoernig (2007) 

  Biotechniques 43, 324