Regulatory

Part:BBa_K511000

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-25)
Revision as of 03:53, 29 September 2011 by Jchien (Talk | contribs)

Hef1a-LacOid Promoter MammoBlock

This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor.

Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.
To see further information about this part, consult the Results found on the iGEM 2011 wiki. Sequence and Features BBa_K511000 SequenceAndFeatures

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Parameters
n/aHef1a-LacOid Promoter MammoBlock