Part:BBa_K511000

Hef1a-LacOid Promoter MammoBlock

This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor.

Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.

To see further information about this part, consult the Results found on the iGEM 2011 wiki. [[http://2011.igem.org/Team:MIT/Results/#cmv-to]

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 416
  Illegal PstI site found at 921
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 292
  Illegal PstI site found at 416
  Illegal PstI site found at 921
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 670
  Illegal BamHI site found at 255
  Illegal XhoI site found at 1069
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 416
  Illegal PstI site found at 921
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 416
  Illegal PstI site found at 921
  Illegal NgoMIV site found at 804
  Illegal AgeI site found at 80
  
• 1000
  COMPATIBLE WITH RFC[1000]