Conjugation

Part:BBa_K640002:Design

Designed by: Emily Hicks   Group: iGEM11_Calgary   (2011-09-26)
Revision as of 03:40, 29 September 2011 by Yqi2011 (Talk | contribs) (→‎Design Notes)

oriT - Interspecies origin of transfer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

oriT, or origin of transfer, is a DNA platform which allows for the transfer of plasmid DNA from one organism to another. Relaxases and nickases are responsible for cutting the DNA, host cell polymerases facilitate rolling circle replication of one of the plasmid strands in the host, and rep genes mediate transfer to the receptor organism. This oriT part is an alternate form of BBa_J01003, and allows DNA transfer between E. coli and Pseudomonas.

Source

References