Composite

Part:BBa_K316009:Experience

Designed by: IC 2010 Team   Group: iGEM10_Imperial_College_London   (2010-10-25)
Revision as of 02:34, 29 September 2011 by Jrr299 (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K316009

User Reviews

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BBa_K316009 Penn State 2011 iGEM

Part of our reporter project was largely focused on improving the part developed by Imperial College London iGEM 2010 (BBa_K316007) we began by characterizing their test part K316009. The data for this test, using the standard catechol assay described by their team <a href="http://2010.igem.org/Team:Imperial_College_London/Protocol">here</a> is shown below. For induction 10 ul of 100uM of IPTG was used.

PSUK316009.png This data shows that, while their part is capable of extremely fast conversion of catachol to 2-hydroxymuconate semialdehyde, it is also subject to extreme sensitivity that leads to significant results of color production seen from both induced and un-induced cells.

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