Coding

Part:BBa_K654062

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-09-26)
Revision as of 00:50, 29 September 2011 by Catramp (Talk | contribs)

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Fatty Acyl ACP Reductase with 8-His Tag

sll0209 Fatty Acyl ACP Reductase from Synechocystis sp. PCC 6803 (EC 1.3.1.10). Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction.

The Fatty Acyl-ACP Reductase converts fatty acyl-ACP into fatty aldehydes using NADPH.


2011 Utah State FattyAcylCoAReductase.png


Fatty Acyl-ACP Reductase is used in conjunction with Fatty Aldehyde Decarbonylase to produce alkenes and alkanes, hydrocarbon fuels, in the 2011 Utah_State iGEM project.


2011 Utah State Pathway 3.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1015


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Parameters
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