Intermediate

Part:BBa_K542002

Designed by: Anthony Vuong   Group: iGEM11_Lethbridge   (2011-09-22)
Revision as of 00:35, 29 September 2011 by Dustin (Talk | contribs)

Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)

Made by assembling BBa_K542000 with BBa_I13453.

Note that the pBAD promoter (BBa_I13453) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible).

Intermediate part to assemble the "Co-localization Construct".

This part was characterized in BBa_K542008 in E. coli strain DH5alpha demonstrating this is a functional intermediate in that construct. The data sheet for BBa_K542008 is shown below.

UoflECdatasheet.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None