Coding

Part:BBa_K654059

Designed by: Cody Tramp   Group: iGEM11_Utah_State   (2011-09-26)
Revision as of 00:29, 29 September 2011 by Catramp (Talk | contribs)

Fatty Acyl-CoA Synthetase (Ligase) (fadD from E. coli) with 8-His Tag

FadD Fatty Acyl-CoA Synthetase (Ligase) from E. coli. Contains a 8-Histidine tag immediately before the stop codon to allow gel identification and extraction. The FadD Fatty Acyl-CoA Synthetase (Ligase) converts fatty acids into fatty acyl-CoA using ATP.


2011 Utah State FattyAcylCoASynthetase.png


Fatty Acyl-CoA Synthetase is used in conjunction with Thioesterase to greatly increase the concentration of Fatty Acyl-CoA present in the cell, to increase the amount of bioproducts produced. It is used to produce Fatty Alcohols and Wax Esters in the 2011 Utah_State iGEM project.


2011 Utah State Pathway 2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None