Generator

Part:BBa_K511823

Designed by: Grant Robinson   Group: iGEM11_MIT   (2011-09-28)
Revision as of 00:22, 29 September 2011 by Registry (Talk | contribs)

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Inducible Red Fluorescent Protein Generator (UAS-Gal4-mKate) MammoBlock Device

This MammoBlock composite device produces the monomeric red fluorescent protein mKate when induced with Gal4 transactivator variants and otherwise produces mKate at a low, basal (OFF) level.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 126
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 126
    Illegal NheI site found at 154
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 126
    Illegal XhoI site found at 83
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 126
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 126
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 876
    Illegal SapI.rc site found at 258


[edit]
Categories
Parameters
n/aInducible Red Fluorescent Protein Generator (UAS-Gal4-mKate) MammoBlock Device