Coding

Part:BBa_K566027

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-27)
Revision as of 00:06, 29 September 2011 by Rhodwulf (Talk | contribs)

CcaS

Ccas is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along with its response regulator CcaR, activates promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with absorption of red light.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1235
    Illegal SpeI site found at 1330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1235
    Illegal NheI site found at 606
    Illegal SpeI site found at 1330
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1235
    Illegal XhoI site found at 2252
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1235
    Illegal SpeI site found at 1330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1235
    Illegal SpeI site found at 1330
    Illegal AgeI site found at 1270
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None