Coding

Part:BBa_K620001:Design

Designed by: Caltech iGEM 2011   Group: iGEM11_Caltech   (2011-09-20)
Revision as of 23:54, 28 September 2011 by Ashelton (Talk | contribs) (→‎References)

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WT-F87A (p450)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1219
    Illegal SapI.rc site found at 2242
    Illegal SapI.rc site found at 2842


Design Notes

Had to Quikchange to remove PstI sites within the gene.

Source

This part came from Dr. Frances Arnold at Caltech.

References

  • Lewis, J., Mantovani, Simone M., Fu, Y. (2010). Combinatorial Alanine Substitution Enables Rapid Optimization ofCytochrome P450BM3for Selective Hydroxylation of Large Substrates. ChemBioChem Volume 11, Issue 18, 2502–2505. [http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000565/abstract http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000565/abstract]