Coding
cI434 opt

Part:BBa_K566011

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 22:25, 28 September 2011 by Janmatrey (Talk | contribs)

cI434 repressor from phage 434 optimized for E. coli cI (+LVA)

Optimized cI434 repressor with LVA tag from phage 434, with preferential codon usage for improved expression in E. coli.

Usage and Biology

Repressor protein cI from bacteriophage 434. cI434 has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM434 promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM434.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 436
    Illegal AgeI site found at 447
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None