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BBa_K238013 Glasgow iGEM 2011

We coupled the bluf promoter to YFP and measured intensity after induction with white light. We found that expression showed a 2.4 fold increase in intensity when compared with darkness as a control. The promoter demonstrated leaky expression with no light induction. The image above demonstrates expression of YFP ligated to an RBS and the bluf promoter. The images are taken from two samples, one which was grown in darkness and the other grown with light present. The samples were shaken overnight at room temperature and the intensity of expression was measured. (See graph below) Scale = 15 microns. Fluorescence filter : 515-565nm The graph clearly shows that expression has been significantly induced by white light for expression with the bluf domain. When averaged out, the total induction shows an increase in YFP fluorescence by a 2.4 fold level, however, the promoter does exhibit some leaky expression. This was the only light-induced promoter, out of the 3 that the Glasgow iGEM 2011 team trialled, which demonstrated any measurable increase in expression.

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