Generator

Part:BBa_K652003

Designed by: Darren Zhu   Group: iGEM11_Yale   (2011-08-26)
Revision as of 21:41, 28 September 2011 by AaronHakim1991 (Talk | contribs)

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T7-RBS-eGFP-TmAFP-His-Term

This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Tenebrio molitor antifreeze protein (TmAFP) with a His tag at the N-terminus, followed by a terminator. Note, unlike BBa_K652002, this part does NOT contain a TEV cleavage site between eGFP and TmAFP

More information about this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 864
    Illegal AgeI site found at 903
    Illegal AgeI site found at 975
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None