Coding
cI opt

Part:BBa_K566012

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 20:07, 28 September 2011 by Janmatrey (Talk | contribs)

cI repressor from Lambda phage optimized for E. coli cI (+LVA)

cI repressor from Lambda phage optimized for E. coli. Includes LVA tag.

Preferential codons to improve their expression in E. coli.


Usage and Biology

The lambda repressor is a dimeric protein also known as the cI protein. cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. Modified with a LVA tag for rapid degradation of the protein

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None