Coding

Part:BBa_K590015

Designed by: Michael Brasino, Alicia Wong, Rashmi Ravichandran   Group: iGEM11_Washington   (2011-09-15)
Revision as of 20:04, 28 September 2011 by Robere (Talk | contribs) (→‎Usage and Biology)

sfGFP_mamK_pGA1C3

This part was made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011] and was contributed to the [http://2011.igem.org/Team:Washington/Magnetosomes/Results Magnetosome Toolkit].

Usage and Biology

This part consists of the mamK gene from Magnetospirillum magneticum strain AMB-1, fused to superfolder gfp on pGA1C3. MamK has been reported to be essential for proper magnetosome formation in magnetotactic bacteria; its bacterial actin-like cytoskeleton protein is required for proper alignment of the magnetosomes in a chain. Beyond magnetosome alignment, MamK filaments could act as a scaffold for protein localization or to alter cell morphology by preventing cells from fully dividing (see images below).

This part was transformed into E. coli and fluorescent filament structures were observed. The same construct has been successfully cloned into Gibson assembly vectors pGA1A3 and pGA4C5.

MamK sfGFP 3k3-1 uwigem2011part.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1430
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1430
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1134
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1430
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1430
    Illegal NgoMIV site found at 1156
    Illegal NgoMIV site found at 1523
    Illegal NgoMIV site found at 1670
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 80
    Illegal SapI.rc site found at 1802


[edit]
Categories
Parameters
None