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Regulatory
Part:BBa_K656010:Design
Designed by: Evan Clark, Julius Ho, Eli Moss, Jesse Palmer Group: iGEM11_Brown-Stanford (2011-09-19)
Anabaena pSac Promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
"Anabaena" separates the process of photosynthesis and nitrogen fixation spatially, into two cell types. Heterocysts are cells responsible for forming an anaerobic environment in which nitrogen fixation can occur. The more numerous cell type, vegetative cells, are photosynthetic and capable of producing excess sugars.
The promoter we have modeled our part after is present in the Photo-system I of Anabaena 7120, and therefore its activity is both constitutive and confined to vegetative cells. This was done to confine sucrose secretion to only vegetative cells, as heterocysts are not producing sucrose through photosynthesis.
Source
Anaebaena PCC 7120