Inverter

Part:BBa_K542003

Designed by: Anthony Vuong   Group: iGEM11_Lethbridge   (2011-09-23)
Revision as of 18:47, 28 September 2011 by Liszabruder (Talk | contribs)

pBAD Regulated TetR

Made by assembling BBa_I13453 with BBa_P0440.

Production of TetR is regulated by the pBAD promoter (BBa_I13453). TetR is an inhibitor of the constitutively "on" promoter pTet--BBa_R0040). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")

Intermediate part to assemble the "Co-localization Construct".

Datasheet for the test construct Part BBa_K542008 in E. coli strain DH5alpha demonstrating this is a functional intermediate in that construct.

UoflECdatasheet.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None