Device

Part:BBa_K598020

Designed by: Zhenrun ZHANG   Group: iGEM11_Peking_R   (2011-09-28)
Revision as of 16:34, 28 September 2011 by Registry (Talk | contribs)

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B0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE

B0015+pBAD+vioAB+B0015+pBAD+TPP ribozyme 1.20+vioD+B0015+pBAD+vioE

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 262
    Illegal NheI site found at 1296
    Illegal NheI site found at 4826
    Illegal NheI site found at 6466
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 202
    Illegal BamHI site found at 4766
    Illegal BamHI site found at 6406
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4081
    Illegal AgeI site found at 4277
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 5915
    Illegal SapI.rc site found at 5990


[edit]
Categories
//chassis/prokaryote/ecoli
//direction/forward
//plasmidbackbone/copynumber/high
//regulation/negative
Parameters
chassisE. coli DH5α
ligandsThiamine Pyrophosphate (TPP)
n/aB0015+pBAD+vioAB+B0015+pBAD+TPP Up-regulated Hammerhead Ribozyme 1.20+vioD+B0015+pBAD+vioE
resistance chloramphenicol