Coding

Part:BBa_K620000:Design

Designed by: Caltech iGEM 2011   Group: iGEM11_Caltech   (2011-09-20)
Revision as of 09:21, 28 September 2011 by Ashelton (Talk | contribs) (→‎References)

DDT Dehydrochlorinase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part was assembled through PCR using a set of long oligos. The sequence contains an NdeI site, which made it more difficult to place into a pET11a vector for protein purification and characterization.


Source

This part was isolated from Anopheles dirus. The team took the amino acid sequence found in the NCBI protein database and used reverse transcribed using codon optimization for E. coli to create the DNA part.

References

  • [http://www.ncbi.nlm.nih.gov/protein/Q93113.1 http://www.ncbi.nlm.nih.gov/protein/Q93113.1]
  • Prapanthadara, L., Koottathep, S., Promtet, N., Hemingway, J., Ketterman, A. (1995) Purification and characterization of a major glutathione S-transferase from the mosquito Anopheles dirus (Species B). Insect Biochemistry and Molecular Biology Volume 26, Issue 3, 277-285. [http://www.sciencedirect.com/science/article/pii/0965174895000909 http://www.sciencedirect.com/science/article/pii/0965174895000909]