Composite
CFP

Part:BBa_K566004:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 08:12, 28 September 2011 by Janmatrey (Talk | contribs)

CFP protein generator without transcription terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for better expression in E. coli. Illegal sites were removed.


Source

Synthesized DNA.

References

1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell science at a glance 5:837-838