Other
Part:BBa_K648123:Design
Designed by: Elyse Merkel Group: iGEM11_Penn_State (2011-09-28)
RecA with mutation of Lys 286 and Arg 243
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 127
Illegal AgeI site found at 631 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Mutation of active RecA at two different amino acids to attempt to prevent RecA from repairing damaged DNA but not other functions
Source
RecA 1 DH10B BBa_648100