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Part:BBa_K648123:Design

Designed by: Elyse Merkel   Group: iGEM11_Penn_State   (2011-09-28)
Revision as of 07:21, 28 September 2011 by Registry (Talk | contribs)

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RecA with mutation of Lys 286 and Arg 243


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 127
    Illegal AgeI site found at 631
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mutation of active RecA at two different amino acids to attempt to prevent RecA from repairing damaged DNA but not other functions


Source

RecA 1 DH10B BBa_648100

References