Composite
CFP

Part:BBa_K566004:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 05:32, 28 September 2011 by Janmatrey (Talk | contribs)

CFP protein generator without transcription terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 39
    Illegal XhoI site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for better expression in E. coli. Illegal sites were removed.


Source

Synthesized DNA.

References

1. Patterson G., Rich N. Day and David P.(2001) Fluorescent protein spectra. Cell science at a glance 5:837-838