Measurement

Part:BBa_K648034:Experience

Designed by: Lauren Rossi, Jamie Coletta, Anisha Katyal, Kristen Salava   Group: iGEM11_Penn_State   (2011-09-27)
Revision as of 02:58, 28 September 2011 by Ler172 (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K648034

User Reviews

We used this part to test that the promoter on the right side of Or was working properly. The production of red fluorescence protein was measured using a TECAN machine, and compared to a normal strand of E. coli. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional. A graph of the results is shown below.

PSUOrTest.png



UNIQc2aef45ba917b966-partinfo-00000000-QINU UNIQc2aef45ba917b966-partinfo-00000001-QINU