Regulatory
OL

Part:BBa_K566000

Designed by: Daniel Rodriguez, Eduardo Almeyda, Porfirio Quintero   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 01:33, 28 September 2011 by Quintero (Talk | contribs)

OL region from Lambda

OL region from Lambda phage. Allows biphasic switch (K566002) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter (K566001).

Usage and Biology

OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda (1). Its binding sites maybe used to design of new regulatory networks or to mimic Lambda's natural behavior.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation/multiple
Parameters
None