Regulatory
OL

Part:BBa_K566000:Design

Designed by: Daniel Rodriguez, Eduardo Almeyda, Porfirio Quintero   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 01:23, 28 September 2011 by Quintero (Talk | contribs) (→‎Design Notes)

OL region from Lambda


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original sequence does not have any illegal restriction sites.

Source

Obtained by PCR directly from Lambda genomic DNA.

References

1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.

2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.