Composite

Part:BBa_K566006

Designed by: Daniel Rodriguez and Porfirio Quintero   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)
Revision as of 19:51, 27 September 2011 by Quintero (Talk | contribs) (→‎Usage and Biology)

penI-pCpcG2-pPenI-mnt

Repression-based potential AND gate. Mnt Repressor is under the control of PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter.

Usage and Biology

This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter (BBa_R0073 ); so that only when green light is ON and any other Mnt source is OFF, expression from pMnt is allowed. The other Mnt source must then be placed under the control of a repressible promoter (other than pPenI) whose repressor expression is controlled by the second input.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 992
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 912
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//regulation/multiple
Parameters
None