DNA

Part:BBa_K676009:Design

Designed by: Kheng Tee Ng   Group: iGEM11_UCL_London   (2011-09-17)
Revision as of 17:49, 27 September 2011 by Khengtee (Talk | contribs) (→‎Design Notes)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Gyrase B


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 428
    Illegal BglII site found at 1667
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 100
    Illegal AgeI site found at 238
    Illegal AgeI site found at 931
    Illegal AgeI site found at 2047
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The ORF has a EcoR1 restriction site on the 6th bp position , thus a site directed mutagenesis has been carried out to remove the site.

Source

The ORF comes from E.coli.

References