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Composite

Part:BBa_K539461

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)
Revision as of 17:48, 27 September 2011 by Zcc (Talk | contribs)

promoter (lacI regulated)+RNA thermometer+vioD with RNA thermometer+tetR+double terminator

Built in Plac promoter(Part:BBa_R0010), followed by vioD(Part:BBa_K539413), 37’celcius regulator RBSs, tetR and terminator(Part:BBa_K332033). It catalyze protodeoxyviolaceinic acid(PVA) into protoviolaceinic acid and inhibit Ptet contained circuit when the temperature hit 37’C or higher.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1194
    Illegal SapI.rc site found at 1269


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Categories
Parameters
n/apromoter (lacI regulated)+RNA thermometer+vioD with RNA thermometer+tetR+double terminator