Reporter
Part:BBa_K634014
Designed by: Eric Walters Group: iGEM11_Wisconsin-Madison (2011-09-27)
Reversed J23100-alkS
This part was used as a component of an alkane sensor which utilized the transcription factor AlkS acting upon the promoter PalkB to express the red fluorescent protein tagRFP.
Note: This part is on the antisense strand, and can be considered reversed. Keep this in mind if cloning downstream of alkS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 731
Illegal NgoMIV site found at 767
Illegal AgeI site found at 2046 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |